The Gel Doc EZ imager is designed and certified to meet EN, the internationally accepted electrical safety standard, and EN Class A EMC regulations. Certified products are safe to use when operated in accordance with the instruction manual. This instrument should not be mo dified or altered in any way. Modification or alteration of. • Please read the instruction manual carefully. • The instrument must be used only for the intended purpose of gel documentation in research laboratories. • The instrument must be connected to a grounded power source line and protected by a circuit breaker. • . Instruction Manual and Application Guide Required Materials n Criterion™ precast gels n Criterion or Criterion™ Dodeca™cell n PowerPac™ Basic or PowerPac HC power supply (or equivalent) n Sample buffer n Running buffer ( ml per gel) Setting Up and Running Criterion Gels in .
Criterion™ Gel More Data One Gel. Criterion™ Cassette Features. Selection of Precast Gels. Precast Gel Comb Styles. Criterion precast gels include a broad selection of mid-sized polyacrylamide gels in cassettes with integrated upper chambers. Individually packaged in various formulations, these gels can run up to 26 samples. 2. Fix gel 2 × 15 minutes in 30% ethanol% acetic acid solution. 3. Wash gel 2 × 5 minutes in 10% ethanol, then 2 × 5 minutes in ultrapure water. 4. Prepare Sensitizer Working Solution (50µL Sensitizer with 25mL water). 5. Sensitize gel for 1 minute, then wash 2 × 1 minute with water. 6. Criterion ™ Precast Gels Instruction Manual and Application Guide. Bio-Rad Technical Support. For help and technical advice, please contact the Bio-Rad Technical Support department. In the United States, the Technical. Support department is open Monday–Friday, am – pm, Pacific Time. Phone: Fax:
Criterion XT precast gels are formulated at a near-neutral pH, significantly delaying acrylamide hydrolysis compared to traditional Laemmli systems. Their. a Criterion 4–20%. Tris-HCl gel and Gel Blot. Instruction Manual. Precision Plus. Protein. WesternC neutral pH gels (Criterion XT gels or similar). handcast gels with the Criterion cell and Criterion XT precast gels Mini-PROTEAN Gel Instruction Manual and Application Guide, online.
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